Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA-guided FokI nucleases (RFNs ...
Figure 1: Architectures of Cas9 and FokI-dCas9 fusion variants. We chose two sets of sgRNA binding-site pairs with different orientations within GFP. One set placed the pair of NGG PAM sequences ...
Developments in molecular diagnostics have been vital in discovering novel pathogens and diagnosing many human diseases. The recent coronavirus 2019 (COVID-19) pandemic, caused by severe acute ...
On the grounds of Roland Garros this week, a Spanish terrier-bulldog mix has been turning heads and warming hearts. They call him Foki. He’s anything but poky when darting around the court chasing ...
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